南方农业学报2013，Vol.44Issue(7)：1076-1080,5.DOI:10.3969/j:issn.2095-1191.2013.7.1076
特异性检测植物乳杆菌的多重PCR方法
A specific method for detection of Lactobacillus plantarum by multiplex PCR
摘要
Abstract
[Objective]The present study was conducted to establish a specific method for detection of Lactobacillus plantarum without any interference.[Method]Lactobacillus plantarum specific sequences were screened by SMM system,and multiplex PCR detection of different dairy products was conducted based on the designed primer of specific sequences.[Result]The designed seven primers performed good specificity,among which three primers,free from the interference of Lactobacillus pentosus and Lactobacillus plantarum,could produce specific sequences of 101 bp (Primer LP-1 & LP-2),189 bp (Primer LP-5 & LP-6) and 378 bp (Primer LP-9 & LP-10).The limit of detection was 2.2×10 CFU/mL.Using multiplex PCR and agarose gel electrophoresis to test self-made yogurt products with lactic acid bacteria,natural fermented products,and yogurts commodities,Lactobacillus plantarum could be detected to distinguish kindred strains of Lactobacillus plantarum.[Conclusion]Due to its low cost,simple procedures,brief time,high sensitivity and specificity,this method should be used for fast detection of Lactobacillus plantarum in actual production.关键词
植物乳杆菌/SMM系统/多重PCR/定性检测Key words
Lactobacillus plantarum/ SMM system / multiplex PCR / qualitative detection分类
轻工纺织引用本文复制引用
陈臣,周方方,任婧,刘振民,陈卫..特异性检测植物乳杆菌的多重PCR方法[J].南方农业学报,2013,44(7):1076-1080,5.基金项目
"十二五"国家863计划项目(2011AA100901) （2011AA100901）
