南方农业学报2013，Vol.44Issue(7)：1081-1086,6.DOI:10.3969/j:issn.2095-1191.2013.7.1081
农杆菌介导的葡萄柚转化再生体系建立研究
Establishment regeneration system for Citrus.paradisi Macf.cv Duncan by Agrobacterium-mediated transformation
摘要
Abstract
[Objective]Transformation regeneration system of epicotyls derived from Citrus paradisi Macf.cv Duncan seedling were established in order to provide useful reference for its gene transformation.[Method]Epicotyls of seedling were used as explants to investigate the influence factors on induction and differentiation of adventitious buds,rooting of plantlets,and genetic transformation.[Result]The induction rate of adventitious buds derived from epicotyles were ranged from 72.7％ to 81.6％ in media with 0.50-1.00 mg/L TDZ.Comparing three types of growth regulators,the higher rooting rate (81.3％) were observed in 1/2MT medium with 1.0 mg/L of ABT 6 rooting powder.Among different antibiotics,timentin showed better sterilizing effects than cefotaxime sodium and ceftriaxone sodium on agrobacterium-mediated transformation plantlets culture.Timentin had no inhibition on buds induction of explants.During initial transformation stage of buds,5-7 days of darkness culture was beneficial to induce Kan anti-buds.Among 7 transgenic plantlets,6 plantlets showed positive in PCR detection.[Conclusion] Regeneration system of Citrus paradisi Macf.cv Duncan ecopityls was established and provided base on its genetic transformation.关键词
葡萄柚/不定芽/再生/转化Key words
Citrus paradisi Macf.cv Duncan/ adventitious bud/ regeneration / transformation分类
农业科技引用本文复制引用
韩美丽,陆荣生,林睿,马跃峰,杨玉霞,覃建林..农杆菌介导的葡萄柚转化再生体系建立研究[J].南方农业学报,2013,44(7):1081-1086,6.基金项目
广西农业科学院基本科研业务专项项目[200808Z(基)] （基）
