南方农业学报2013，Vol.44Issue(7)：1087-1091,5.DOI:10.3969/j:issn.2095-1191.2013.7.1087
马褂木ISSR-PCR扩增体系的优化
Optimization of ISSR reaction system of Liriodendron chinese
摘要
Abstract
[Objective]A stable ISSR reaction system suitable for Liriodendron chinese was established to provide technical support for ISSR diversity analysis of Liriodendron chinese population.[Method]In the single factor experiment,main factors of ISSR reaction system including MgCl2,dNTPs,primer concentration,Taq DNA polymerase,DNA template amount and annealing temperature were optimized and screened.[Result]Using kit to extract DNA in Liriodendron chinese leaves was better than using the improved CTAB method.The optimal PCR reaction system was:20 μL reaction volume containing 2.0 μL10× buffer,1.8 mmol/L MgC12,0.2 mmol/L dNTP,05 μmol/L primer,1U Taq polymerase,60 ng template DNA,and 59.1℃ annealing temperature.[Conclusion]Through the optimized ISSR-PCR reaction system,clear and stable stripes could be attained,thus it's proper for ISSR diversity analysis of Liriodendron chinese population.关键词
马褂木/ISSR/扩增体系/优化Key words
Liriodendron chinese/ ISSR/ reaction system/ optimization分类
农业科技引用本文复制引用
石晓蒙,谭飞燕,黄寿先,蒙奕奕,黄家华..马褂木ISSR-PCR扩增体系的优化[J].南方农业学报,2013,44(7):1087-1091,5.基金项目
广西科技攻关项目(桂科攻10100012-2) （桂科攻10100012-2）
广西"十一五"林业科技项目(林科字2009[16]) （林科字2009[16]）
