河南农业大学学报2013，Vol.47Issue(3)：268-271,288,5.
马铃薯C-8,7甾醇异构酶基因原核表达载体的构建和表达
Construction of prokaryotic expression vectors for StSI1 and its induced expression
摘要
Abstract
Two prokaryotic expression vectors pGEX-StSI1 b and pGEX-StSI1 c for the potato StSI1 (C-8,7 sterol isomerase) gene cDNA StSI1 both with and without signal peptide were constructed and the induced expression conditions for the fusion protein GST-StSI1b and GST-StSI1c were optimized in the engineered Escherichia coli BL21 (DE3).The results showed that the fused GST-StSI1b and GSTStSI1c proteins could be effectively expressed under different concentrations of IPTG,including 0.1,0.5 and 1.0 mmol · L-1,and the most suitable concentration of IPTG was 1.0 mmol · L-1.As to the induction time,the fusion protein began to express after 3 h of induction under the 3 different IPTG concentrations,and its expression abundance increased along with the induction time and reached to the highest point at the 9 h of induction.In general,the most suitable induction condition for the fusion protein was 9 h induction under 1.0 mmol · L-1 IPTG.关键词
马铃薯/甾醇异构酶/原核表达Key words
potato/ StS(I1)/ prokaryotic expression分类
农业科技引用本文复制引用
白润娥,邓利,曹玲珑,李冬兵,熊大斌,牛洪斌,尹钧..马铃薯C-8,7甾醇异构酶基因原核表达载体的构建和表达[J].河南农业大学学报,2013,47(3):268-271,288,5.基金项目
河南省科技攻关项目(30200302) （30200302）
