实用妇产科杂志2013，Vol.29Issue(8)：613-617,5.
妊娠期高血压疾病特异性标志物的蛋白组学测定及应用
Proteomics Determination and Application of Tumor-Specific Markers in Hypertension Disorders Complicating Pregnancy
摘要
Abstract
Objective:To explore the serum proteomic fingerprints of patients with Late-onset preeclampsia and try to provide experimental basis for the early prediction of gestational hypertension disease.Methods:Fifteen patients with late-onset preeclampsia(late-onset preeclampsia group) and fifteen healthy pregnant women (normal control group)and fifteen Hypertension (non-pregnant hypertension group)were tested by surface-enhanced laser desorption / ionization-time of flight-mass spectrometry (SELD-ITOF-MS).Biomarker Wizard and Biomarker Pattern software were used to detect protein peaks which were significantly different between HDCP and controls,and to establish a primary diagnostic model of HDCP.According to the model,late-onset preeclampsia (21 cases) and normal pregnant women (47 cases) were blind screening and the diagnosed characteristics was also analyzed.Results:①Four protein peaks were significantly different at the molecular range of 2000 ～ 50000 between in gestational hypertension group and in control group (P ＜ 0.05).Sixteen protein peaks were significantly different between in gestational hypertension group and control group (P ＜ 0.05).②Two proteins molecules (5650.40,13991.0 respectively) were screened as a proteomic model,the predictive sensitivity was 69.6％ (16/23),specific degrees was 85.1％ (40/47),accuracy was 82.4％ (56/68).Conclusions:The differently expressed proteins in serum screened by SELD-ITOF-MS could act as the biomarkers of the pregnant hypertension's occurrence and development.The diagnostic pattern built in this study maybe useful for early diagnosis of HDCP.关键词
妊娠期高血压疾病/晚发型子痫前期/生物学标记/蛋白组学/表面增强激光解析电离飞行时间质谱Key words
Hypertension disorders in pregnancy/ Late onset preeclampsia/ Biological markers/ Proteome/Surface-enhanced laser desorp tion/ionization-time of flight-mass spectrometry分类
医药卫生引用本文复制引用
朱文秀,蔡雁,肖巍,卫丽,王晓燕,韩世愈,朱莉,高晓旭,宋敬..妊娠期高血压疾病特异性标志物的蛋白组学测定及应用[J].实用妇产科杂志,2013,29(8):613-617,5.基金项目
黑龙江省自然科学基金(编号:C03030402) （编号:C03030402）
