非洲猪瘟病毒VP73结构蛋白表达及鉴定OA北大核心CSTPCD
Expression and identification of structural protein VP73 of African swine fever virus
根据GenBank登录的非洲猪瘟病毒(ASFV) VP73基因序列,人工合成VP73主要抗原表位区基因片段,克隆至pUC57载体中.设计1对引物,PCR扩增获得429 bp的VP73基因片段；将VP73基因片段使用EcoRI和SalI酶切后亚克隆至表达载体pGEX-KG,经PCR、酶切、测序鉴定挑选阳性克隆,转化至表达菌株BL21 (DM3)中,进行体外诱导表达,SDS-PAGE和Western blot分析蛋白表达及其活性.结果显示,成功构建表…查看全部>>
According to the gene sequence of African swine fever virus (ASFV) VP73 in the GenBank,the gene fragment of major antigenic epitope of VP73 was synthesized and cloned into pUC57 vector.The VP73 gene of ASFV amplified from recombinant pUC57 by PCR,and the product was a 429 bp DNA fragment.The purified VP73 gene fragment was subcloned into pGEX-KG vector by EcoRI and SalI enzymes digestion.The recombinant plasmid was identified by PCR and enzymes digestion.It …查看全部>>
董林;王艳萍;张春玲;沈志强
山东省滨州畜牧兽医研究院,山东滨州256600山东绿都生物科技有限公司,山东滨州256600山东省滨州畜牧兽医研究院,山东滨州256600山东绿都生物科技有限公司,山东滨州256600
农业科技
非洲猪瘟病毒VP73表达
African swine fever virus (ASFV) VP73 expression
《畜牧与兽医》 2013 (8)
23-26,4
山东省自然科学基金资助项目(ZR2010CQ012)
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