定性测定羊奶中黄曲霉毒素M1残留的高灵敏度检测卡的研制OA北大核心CSTPCD
Development of detection card for the qualitative determination of aflatoxin M1 in ewe's milk
为研制定性测定羊奶中黄曲霉毒素M1残留的高灵敏度检测卡,在硝酸纤维素膜上预包被黄曲霉毒素M1全抗原作为检测线,在硝酸纤维素膜上预包被羊抗鼠二抗作为质控线.将抗黄曲霉毒素M1单克隆抗体用胶体金标记作为金标垫.将样品垫、金标垫、预包被黄曲霉毒素M1的硝酸纤维素膜以及吸水垫依次粘贴在PVC板上,切割成4 mm宽的试纸条,将试纸条装入塑料卡壳中扣紧组装成检测卡.样本中残留的黄曲霉毒素M1与硝酸纤维素膜上的检测线共同竞争胶体金标记的黄曲霉毒素M1抗体,检测…查看全部>>
This study is aimed to develop a kind of sensitive detection card for the qualitative determination of aflatoxin M1 residues in milk.The aflatoxin M1 antigen precoated on nitrocellulose membrane was used as detection line,with goat anti-mouse antibody as control line.The anti-aflatoxin M1 monoclonal antibody was labeled on colloidal gold as gold labeled pad.The sample pad,gold labeled pad,nitrocellulose membrane and an absorbent pad were attached on the PVC …查看全部>>
武玉香;沈志强;唐士云
山东绿都生物科技有限公司,山东滨州256600山东绿都生物科技有限公司,山东滨州256600山东滨州畜牧兽医研究院,山东滨州256600
农业科技
黄曲霉毒素M1羊奶检测卡
aflatoxin M1 ewe's milk detection card
《畜牧与兽医》 2013 (8)
31-35,5
山东省现代农业产业技术体系羊产业创新团队项目(20120630)
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