中国普通外科杂志2013，Vol.22Issue(8)：988-993,6.DOI:10.7659/j.issn.1005-6947.2013.08.006
microRNA let-7a对胆管癌细胞凋亡和周期的影响
Effect of let-7a microRNA on apoptosis and cell cycle of cholangiocarcinoma cells
周璐璐 1李灼日1
作者信息
- 1. 海南省人民医院肝胆外科,海南海口570100
- 折叠
摘要
Abstract
Objective:To investigate the let-7a microRNA (miRNA) expression in cholangiocarcinoma tissue and its significance.Methods:The let-7a expressions in tissues of cholangiocarcinoma and normal bile duct specimens were determined by Real-time PCR technique.The cholangiocarcinoma HuCCT-1 cells were divided into let7a overexpression group (transfected with let-7a mimics),negative control group (transfected with negative control sequence) and blank control group (without any transfection),and then the let-7a expression,apoptosis and cell cycle in each group of cells were determined by Real-time PCR and flow cytometer,respectively.Results:The let-7a expression was significantly down-regulated in cholangiocarcinoma tissue than that in normal bile duct tissue (P＜0.01).Both let-7a expression and apoptotic rate in cells of let-7a overexpressionObjective:group were all significantly increased compared with blank control group (both P＜0.01),while no such differences were noted between the negative control group and blank control group (both P＞0.05).The difference in cell cycle showed no statistical significance among groups (P＞0.05).Conclusion:The let-7a expression is down-regulated in cholangiocarcinoma tissue and inhibits cell apoptosis and,may thereby promote the occurrence and development of cholangiocarcinoma.关键词
胆管肿瘤/微RNAs/细胞凋亡Key words
Bile Duct Neoplasms/ MicroRNAs/ Apoptosis分类
医药卫生引用本文复制引用
周璐璐,李灼日..microRNA let-7a对胆管癌细胞凋亡和周期的影响[J].中国普通外科杂志,2013,22(8):988-993,6.
