中国实用妇科与产科杂志2013，Vol.29Issue(8)：657-661,5.
卵巢上皮性癌中表达Tie2单核巨噬细胞的研究
Study on Tie2-expressing monocytes/macrophages in patients with epithelial ovarian cancer
摘要
Abstract
Objective To investigate the frequency of Tie2-expressing monocytes (TEMs) in the peripheral blood and peritoneum of epithelial ovarian cancer.Methods Between Jul.2011 and Dec.2012,monocytes in peripheral blood were isolated from female healthy donors,patients with benign ovarian tumor and patients with EOC.The mRNA expression of Tie2 was measured by RT-PCR.Flow cytometry was used to detect the rate of TEMs in CD14 + monocytes in peripheral blood.The infiltration of TEMs in peritoneum were detected by immunofluorescence.Results The mRNA and protein of Tie2 expression were identified in monocytes isolated from female healthy donors,patients with benign ovarian tumors and EOC.The frequency of TEMs ranged from 1.82％ to 23.45％ (9.41 ± 5.93) ％ in benign ovarian tumors and 1.71％ to 15.03％ (7.99 ±3.90)％ of CD14+ PBMC (peripheral blood monocytes) in EOC patients.Compared with 1.18％ ～9.56％ (4.13 ±2.86) ％ in healthy donors,the frequency of TEMs was significantly higher in patients with benign ovarian tumors and EOC (All P ＜ 0.05).It was observed that the infiltration of TEMs [(15.17 ± 8.84) ％ (2.27％ ～25.81％)] in the peritoneum of EOC patients.In the mean time,no TEMs was shown in peritoneum in benign ovarian tumors.Conclusion TEMs could be detected in the peripheral blood and peritoneal of EOC patients.The frequency of TEMs was significantly higher in peripheral blood and peritoneal of EOC patients.关键词
Tie2/单核细胞/表达Tie2单核巨噬细胞/卵巢肿瘤Key words
Tie2 / monocyte/ Tie2-expressing monocytes/macrophages / ovarian tumor分类
医药卫生引用本文复制引用
吴丽,王凯,吴小丽,胡婧,赵肖波,汪希鹏..卵巢上皮性癌中表达Tie2单核巨噬细胞的研究[J].中国实用妇科与产科杂志,2013,29(8):657-661,5.基金项目
国家自然基金资助项目(81072136)和上海市科委课题资助项目(10411969200) （81072136）
