肿瘤防治研究2013，Vol.40Issue(7)：643-647,5.DOI:10.3971/j.issn.1000-8578.2013.07.004
铜绿假单胞菌注射液诱导人胰腺癌细胞PANC-1的凋亡
Experimental Study on Inducing Apoptosis of Human Pancreatic Carcinoma Cells PANC-1 by Pseudomonas Aeruginosa Vaccinein in vitro
包丽颖 1孙建民 1张玉宝1
作者信息
- 1. 150000 哈尔滨,哈尔滨医科大学附属第三医院肝胆胰外科
- 折叠
摘要
Abstract
Objective To investigate the inhibitory effects of Pseudomonas aeruginosa vaccine (PAMSHA) on the proliferation of human pancreatic cancer cells and explore the possible mechanism.Methods MTT assay was used to determine the cellgrowth of human pancreatic cancer cell line panc-1 in vitro treated with the vaccine.Inhibition of cellproliferation,changes of super-microstructure and apoptosis of pane-1 were observed by scanning electron microscopy and Tunel technique.The apoptosis rates of the cells from the PA-MSHA group and the control group (the drug concentration was 0) were detected by flow cytometry.The expression levels of apoptosis proteins were evaluated by Western blot.Results PA-MSHA treatment significantly suppressed the proliferation of panc-1 cells from the PA-MSHA group and the control group in a time-and concentration-dependent manner compared with the control group (P =0.006).PA-MSHA vaccine could induce the decrease in number and size,destruction of cell membrane an microvilli,vacuole in cytoplasm and deep staining of nucleus.The expressions of the apoptosis protein caspase-3,caspase-7,caspase-8,caspase-9 and PARP up-regulated in PA-MSHA-treated cells.Conclusion PA-MSHA can suppress the proliferation of pancreatic carcinoma cell in vitro by promoting cell apoptosis.关键词
胰腺癌/凋亡/PANC-1/铜绿假单胞菌Key words
Pancreatic cancer/ Apoptosis/ PANC-1 / Pseudomonas aeruginosa vaccine分类
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包丽颖,孙建民,张玉宝..铜绿假单胞菌注射液诱导人胰腺癌细胞PANC-1的凋亡[J].肿瘤防治研究,2013,40(7):643-647,5.
