肿瘤防治研究2013，Vol.40Issue(7)：648-651,4.DOI:10.3971/j.issn.1000-8578.2013.07.005
LPS对宫颈癌细胞HMGB1主动释放及侵袭转移能力的影响
Effect of LPS Stimulation on Active Release of HMGB1 in Cervical Cancer Cells and Ability of Invasion and Migration
王景 1蔡红兵 2李伟 3李著艳2
作者信息
- 1. 441021 襄阳,襄阳市中心医院(湖北文理学院附属医院)妇产科
- 2. 武汉大学中南医院妇瘤科
- 3. 襄阳市第一人民医院妇产科
- 折叠
摘要
Abstract
Objective To investigate the effect of LPS stimulation on the active release of HMGB1 in Cervical cancer cells and the ability of invasion and migration.Methods Three cell lines were cultured in DMEM:HeLa cell lines,C33A cell lines and HUCEC cell lines (Normal cervical epithelial cells).Western blot was used to detect the intracellular and extracellular protein expression changes of HMGB1 before and after LPS added to the three cell lines.Transwell assay was used to compare the ability of invasion and migration before and after LPS added.Results After stimulated with 100 ng/ml LPS,intracellular total protein HMGB1 all decreased in the three cells,while the expression of HMGB1 in the supernatant increased(P＜0.05) ;the ability of invasion and migration in HeLa and C33A cancer cells significantly enhanced (P＜0.05).Conclusion LPS could facilitate the active release of HMGB1 in cervical cancer cells,and therefore enhanced the ability of invasion and migration.关键词
脂多糖/高迁移率族蛋白1/宫颈癌细胞/肿瘤侵袭Key words
Lipopolysaccharide(LPS) / High-mobility group box-1 /Cervical cancer cells/Tumour cell invasion分类
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王景,蔡红兵,李伟,李著艳..LPS对宫颈癌细胞HMGB1主动释放及侵袭转移能力的影响[J].肿瘤防治研究,2013,40(7):648-651,4.
