安徽农业大学学报2013，Vol.40Issue(3)：464-469,6.
茶树中精氨酸脱羧酶基因的cDNA克隆及序列分析
Cloning and sequence analysis of arginine decarboxylase gene from Camellia sinensis
摘要
Abstract
The full-length cDNA of arginine decarboxylase (ADC,GenBank accession No.JQ65327) gene was obtained by SMART RACE PCR method.It is 2 988 bp in length with an open reading frame (ORF) of 2 163 bp,encoding 720 amino acids with deduced molecular weight of 77.430 kDa and theoretical pI value of 5.373.Bioinformatics analysis show that ADC is a hydrophilic and unstable protein without any signal peptide,has no winded helix structure and transmembrane domain.There are 41 phosphorylation sites within the polypeptide chain.ADC is non-secreted protein and it functions in chloroplast stroma.The sequence analysis of ADC gene would bring some new clues for further exploration of the function of ADC in nitrogen metabolism and theanine biosynthesis.关键词
茶精氨酸脱羧酶/全长cDNA/基因克隆/序列分析Key words
arginine decarboxylase/ full-length cDNA/ gene cloning/ sequence analysis分类
农业科技引用本文复制引用
徐乾,史成颖,宛晓春,汤志近..茶树中精氨酸脱羧酶基因的cDNA克隆及序列分析[J].安徽农业大学学报,2013,40(3):464-469,6.基金项目
国家自然科学基金(31170283),教育部博士点基金(20113418130001)和安徽省自然科学基金(KJ2010A106)共同资助. （31170283）
