草业学报2013，Vol.22Issue(4)：205-211,7.DOI:10.11686/cyxb20130425
外源性金属硫蛋白对中国荷斯坦奶牛血淋巴细胞凋亡/坏死及线粒体膜电位的影响
Effects of exogenous metallothionein on blood lymphocytes apoptosis and mitochondrial transmembrane potential of China Holstein cows
摘要
Abstract
Effects of exogenous metallothionein (MT) on lymphocytes apoptosis,necrosis and mitochondrial membrane voltage (△Ψm) of China Holstein cows were studied using 20 lactating cows in heat stress.They were randomly allocated to groups A,B,C and D.MT was supplemented at 0.0,4.0,8.0 and 16.0 mg/capita,respectively,by intravenous injection.Corrected milk yields in groups B,C,and D were significant higher than those in group A after being injected MT during days 51-60 (P＜0.05); at 35 d,the lymphocytes apoptosis rate of group C (8 mg/capita) was significantly lower than those of groups B and D (P＜0.05),but was not significantly different from group A (P＞0.05).The lymphocytes apoptosis rate of group D (16 mg/capita) was the highest at 35 d (P＜0.05) but significantly (P＜0.05) decreased during days 50-60; Compared to 1 d,the lymphocytes necrosis rate of group A significantly (P＜0.05) decreased at 50 d,group C (8 mg/capita) significantly (P＜0.05) decreased at 35 d and 50 d,and began to show a rising trend at 60 d (P＞0.05),while group D (16 mg/capita) significantly decreased at 50 d and 60 d.The mitochondrial membrane voltage (△Ψm) of group A was the highest at 35 d,but the △ Ψm of group B rose fastest at 35 d (P＜0.05),and was significantly higher than at 20 d (P＜0.05).The △Ψm of groups A and B decreased at 50 d with that of group A falling fastest.The △Ψm of group C was constant during days 35-50 but increased slightly at 60 d.The △Ψm of group D (16 mg/capita) increased gradually during days 1-35 and remained constant during days 51-60.The lymphocytes △Ψm and injected MT amount were positively correlated at 60 d.In conclusion,the heath of cows under heat stress can be improved,by exogenous MT injection.The injection of 8-16 mg/capita,can inhibit the lagged effects of southern summer heat stress in lactating cow.关键词
金属硫蛋白/中国荷斯坦奶牛/细胞凋亡/细胞坏死/线粒体膜电位Key words
metallothionein/ China Holstein cows/ apoptosis/ necrosis / mitochondrial membrane voltage分类
农业科技引用本文复制引用
吴力专,罗佳捷,张彬,李丽立,罗锐,占今舜..外源性金属硫蛋白对中国荷斯坦奶牛血淋巴细胞凋亡/坏死及线粒体膜电位的影响[J].草业学报,2013,22(4):205-211,7.基金项目
国家自然科学基金项目(31072053 （）
30671516),湖南省自然科学基金重点项目(11JJ2014),教育部高等学校博士学科点专项科研基金(博导类)项目(20104320110001)和湖南省博士生科技创新基金项目(CX2010B284)资助. （11JJ2014）
