Journal of Changshu Institute of TechnologyIssue(4)：52-56,5.
松江鲈 MyoD 基因 cDNA部分片段的序列和表达特征分析
Identification and Characterization of Partial cDNA Sequence of MyoD Gene in Trachidermus fasciatus
摘要
Abstract
Myogenic determination gene is an important member of the myogenic regulation factors (MRF) family, which plays an important role in the specific gene transcription. A 957 bp long MyoD cDNA sequence in Trachider⁃mus fasciatus was obtained by using RT-PCR and 3′- RACE method, which included a 390 bp 3′-UTR, and par⁃tial ORF 567bp encoding 188 amino acid peptide. MyoG gene expression was detected in all six examined tissues, and highly expressed in heart. It suggested that the MyoD gene might contribute to a wide range of biological func⁃tions.关键词
松江鲈/生肌决定因子/3’-RACE/组织表达Key words
Trachidermus fasciatus/MyoD/3′-RACE/tissue expressional pattern分类
农业科技引用本文复制引用
蔡宝平,李风鸣,郑红梅,杨磊,邵芳,卢祥云,顾志良..松江鲈 MyoD 基因 cDNA部分片段的序列和表达特征分析[J].Journal of Changshu Institute of Technology,2012,(4):52-56,5.基金项目
2011年江苏省大学生实践创新训练计划项目“松江鲈肌肉调节因子基因克隆和表达特征研究” （）
常熟市科技项目“松江鲈种质资源的保存和优质基因的发掘利用”(CN201111) （CN201111）
