动物营养学报2013，Vol.25Issue(8)：1775-1784,10.DOI:10.3969/j.issn.1006-267x.2013.08.015
日粮中氨化稻草水平对体外培养发酵甲烷和挥发性脂肪酸产量的影响
Effects of Levels of Ammoniated Rice Straw Included in Rations on Rumen Methane and Volatile Fatty Acids Production in in Vitro Incubation
摘要
Abstract
The objective of this experiment was to study the effects of different proportions of untreated and ammoniated rice straw in mixed rations on methane and volatile fatty acids (VFA) production.Two Simmental cattle fitted with rumen cannulas were used as the donors of rumen fluid.Three levels of urea,i.e.0％ (untreated),4％ and 6％ were used for ammoniation treatment of rice straw,respectively.The untreated and ammoniated rice straws were mixed with concentrate mixture at the ratios of 0∶100,20.∶80,40∶60,60∶40,80∶20 and 100∶0,respectively,as experimental treatments.The gas production technique of Menke et al.(1979) was used for in vitro fermentation and the measurement of 48 h VFA,methane and total gas production.The results showed that 48 h total VFA (TVFA) production of the groups with 4％ urea ammoniated rice straw and 6％ urea ammoniated rice straw was significantly higher than that of the group with non-ammoniated rice straw when concentrate/roughage ratio was 0∶100 (P ＜ 0.05).The production of acetate,propionate and TVFA was significantly increased with the increase of concentrate/roughage ratio in the ration (P ＜ 0.05),and acetate/propionate and acetate/TVFA were gradually reduced (P ＜ 0.05).The 48 h methane production of the groups with 4％ urea ammoniated rice straw and 6％ urea ammoniated rice straw was significantly higher than that of the group with non-ammoniated rice straw when concentrate/roughage ratio was 40∶60 (P ＜ 0.05),but 48 h total gas production of the group with 4 ％ urea ammoniated rice straw was significantly higher than that of the group with non-ammoniated rice straw (P ＜ 0.05),and was higher than that of the group with 6％ urea ammoniated rice straw to some extent (P ＞ 0.05).The 48 h methane and total gas production was significantly increased with the increase of concentrate/roughage ratio in the ration (P ＜ 0.05).The best associative effect of 48 h total gas and methane production was found in the group with 4％ urea ammoniated rice straw and concentrate/roughage ratio of 40∶60.The 48 h total gas production in each group had a positive associative effect,but CH4/TVFA in the group with 4％ urea ammoniated rice straw had a negative associative effect.This study supports that methane production is affected by both concentrate/roughage ratio and quality of forage in the ration.The 48 h total gas and the methane production are highest,but CH4/TVFA is the lowest when the ratio of concentrate/4％ urea ammoniated rice straw is 40∶60.关键词
稻草/氨化/瘤胃/甲烷/挥发性脂肪酸Key words
rice straw / ammoniation/ rumen/ methane/ volatile fatty acids分类
农业科技引用本文复制引用
王满红,赵广永..日粮中氨化稻草水平对体外培养发酵甲烷和挥发性脂肪酸产量的影响[J].动物营养学报,2013,25(8):1775-1784,10.基金项目
现代农业产业技术体系北京市奶牛创新团队项目和国家科技支撑计划项目(2011BAD26B03-4) （2011BAD26B03-4）
