发光学报2013，Vol.34Issue(8)：1089-1095,7.DOI:10.3788/fgxb20133408.1089
高灵敏度化学发光免疫法检测人血清中促甲状腺激素
A Highly Sensitive Chemiluminescence Immunoassay Technique for Assaying TSH in Human Serum
齐素文 1张会生 1刘昕宇 1李乔亮1
作者信息
- 1. 深圳大学医学院生物医学工程系,广东深圳518060
- 折叠
摘要
Abstract
A highly sensitive magnetic particle-based chemiluminescence immunoassay (CLIA) technique for assaying thyroid stimulating hormone (TSH) in serum was described.Fluorescein isothiocyanate (FITC) and N-(aminobutyl)-N-(ethylisoluminol) (ABEI) were used to label two different monoclonal antibodies of anti-TSH.Both of the labeled antibodies combined with TSH to form a sandwiched immunoreaction that was monitored by chemiluminescence (CL) detection.The magnetic particles (MPs) that were coated with anti-FITC antibody served as both the solid phase and the separator.The method showed high sensitivty with a detection limit of 0.01 μIU/mL.The coefficient of variation of intra-assay imprecision and the inter-assay imprecision were less than 10％.The average recoveries were 96％ ～ 103％.A correlation analysis against an established automated assay generated a correlation coefficient of 0.993 7.The proposed method demonstrates an acceptable performance for quantifying serum TSH and is suitable for the clinical lab.关键词
促甲状腺激素/异鲁米诺/化学发光免疫分析Key words
thyroid stimulating hormone/ N-(aminobutyl)-N-(ethylisoluminol) / chemiluminescence immunoassay分类
医药卫生引用本文复制引用
齐素文,张会生,刘昕宇,李乔亮..高灵敏度化学发光免疫法检测人血清中促甲状腺激素[J].发光学报,2013,34(8):1089-1095,7.
