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枯萎病生防菌FJAT-4的生长与抑菌作用的温度效应

葛慈斌 刘波 肖荣凤 朱育菁 唐建阳

福建农业学报2013,Vol.28Issue(7):697-704,8.
福建农业学报2013,Vol.28Issue(7):697-704,8.

枯萎病生防菌FJAT-4的生长与抑菌作用的温度效应

Effect of Temperature on the Growth and Antifungal Activity of Fusarium-wilt-disease Biocontrol Bacterium Strain FJAT-4

葛慈斌 1刘波 1肖荣凤 1朱育菁 1唐建阳1

作者信息

  • 1. 福建省农业科学院农业生物资源研究所,福建福州 350003
  • 折叠

摘要

Abstract

To characterize the effect of temperature on the growth and antifungal activity of biological control strain Bacillus licheniformis FJAT-4,the growth response of strain FJAT-4 at different temperature,the effect of concentration and temperature on the antifungal activity of the strain FJAT-4 against Fusarium oxysporum strain FJAT-135 were studied by culturing the strain on plate culture media,liquid media and culturing the strain FJAT-135 on the media contained strain FJAT-4.The results showed that the growth is inhibited and the growth curve is liner type under 20℃,but the growth return to normal and the growth curve is logarithmic type over 25℃,and the optimal temperature is 35-40℃.The cultured liquids of strain FJAT-4 raised at different temperature and its 10 times serial dilution inhibited the growth of the strain FJAT-135 effectively,and the differences among the inhibitions were far less than the differences among the concentrations of the cultured liquid.There were significant differences among effects of temperatures on the inhibitions of FJAT-4 on FJAT-135,the inhibitory effect was the weakest at 15℃,and obvious at 20-30℃ and the highest at 35℃.Therefore,there were significant relationship between the temperatures and the growth and antifungal activity of strain FJAT-4,and the optimal temperature for the growth and antifungal activity was 35-40℃.

关键词

地衣芽胞杆菌/尖孢镰刀菌/抑菌作用/温度效应

Key words

Bacillus licheniformis / Fusarium oxysporum / antifungal activity/ temperature effect

分类

农业科技

引用本文复制引用

葛慈斌,刘波,肖荣凤,朱育菁,唐建阳..枯萎病生防菌FJAT-4的生长与抑菌作用的温度效应[J].福建农业学报,2013,28(7):697-704,8.

基金项目

国家重点基础研究发展计划("973"计划)项目(2011CB111607) ("973"计划)

农业部"引进国际先进农业科学技术"重点项目(2011-G25),福建省财政专项——福建省农业科学院科技创新团队建设项目(STIF-Y03) (2011-G25)

福建农业学报

OACSTPCD

1008-0384

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