福建农业学报2013，Vol.28Issue(7)：709-713,5.
植物蛋白发酵乳酸菌对模拟胃肠道环境的耐受性研究
Tolerance of Lactic Acid Bacteria Strains for Phytoprotein Yoghurt Fermentation to Simulated Gastrointestinal Environments
摘要
Abstract
Previous studies have revealed that Lactobacillus plantarum FJAT-7926 and Lactobacillus casei FJAT-7928 are promising fermentation agents for phytoprotein yoghurt beverage.The aim of our study was to examine the tolerance differences of the two strains under artificial gastric juice with different pH values and artificial intestinal juice containing different concentrates of bile salt and trypsin.The results showed that both of them have high tolerance responses to low pH value.When incubated in artificial gastric juice with pH3.0 for 4 h,survival of each tested strain was over 1×108cfu · mL-1 with a survival rate of 56.06％ and 55.65％,respectively.However,the survival of them declined to 1 × 105 cfu · mL-1 when incubated in the juice with pH2.0 for 2 h,and remained at concentrations of 3.67 × 102 cfu · mL 1 and 2.30 × 103 cfu · mL-1 in incubation for 4 h.Our results suggest that both of the tested strains have great tolerance to the artificial intestinal juice.Though the survival rate of FJAT-7928 and FJAT-7926 decreased as the concentration of bile salt increased,the concentrates of the both strains kept in the same order of magnitude.After 4 h incubation in the artificial intestinal juice containing 0.3％ or 0.5％ bile salt,the survival rates for FJAT-7926 and FJAT-7928 were more than 70％ and 40％,respectirely.The above indicated that both FJAT-7926 and FJAT-7928 have high tolerance to simulated gastrointestinal environments and both of them have broad prospects in the application of fermentation of phytoprotein yoghurt beverage.关键词
植物蛋白/植物乳杆菌/干酪乳杆菌/人工胃液/人工肠液Key words
phytoprotein/ Lactobacillus plantarum / Lactobacillus casei / artificial gastric juice/ artificial intestinal juice分类
生物科学引用本文复制引用
刘芸,曹宜,刘波,刘丹莹,陈倩倩..植物蛋白发酵乳酸菌对模拟胃肠道环境的耐受性研究[J].福建农业学报,2013,28(7):709-713,5.基金项目
福建省科技计划项目——省属公益类科研院所基本科研专项(2011R1020-7) （2011R1020-7）
国家科技部农业科技成果转化资金项目(2013GB2C400236) （2013GB2C400236）
