福建畜牧兽医Issue(4)：4-6,7,4.
鸭疫里默氏菌的分离培养和PCR鉴定
Separation and PCR Identification of Riemerella anatipestifer
陈美昌 1王雪娇 2李巧苹 3王全溪2
作者信息
- 1. 福建省连江县动物疫病预防控制中心 福建连江 350500
- 2. 福建农林大学动物科学学院 福州 350002
- 3. 福州出入境检验检疫局 福州 350001
- 折叠
摘要
Abstract
The clinical symptoms and pathological change were observed from the sick young Muscovy ducks that were got from the farm. Then separation and purification of pathogens were carried on. Meanwhile a pair of specificity primer was designed according to the outside membrane protein conservative district of the bacterium,and the gene was amplified by PCR. Then the purpose sequence was sequenced and analyzed. The results showed that the bacterium was gram-negative ,non-spore-bearing,eubacterium minutum. And the smooth circular transparency bacterial colony was observed on the TSA culture medium. There was fluorescence on the sur-face of the bacterium. The bacterium can not ferment the sugar. According to those specialities,the bacterium was thought to Riemerella anatipestifer. Then a 600 bp fragment was amplified by PCR,and the gene sequence was sequenced. Sequence alignment was done in genebank. According to that the bacterium was identified to Riemerella anatipestifer.关键词
鸭疫里默氏菌/分离培养/PCR鉴定Key words
Riemerella anatipestifer/separation and identification/PCR detection引用本文复制引用
陈美昌,王雪娇,李巧苹,王全溪..鸭疫里默氏菌的分离培养和PCR鉴定[J].福建畜牧兽医,2012,(4):4-6,7,4.
