分析测试学报2013，Vol.32Issue(8)：941-946,6.DOI:10.3969/j.issn.1004-4957.2013.08.006
高效液相色谱-电感耦合等离子体质谱法测定烟草中砷的形态
Determination of Arsenic and Its Species in Tobacco by High Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry
摘要
Abstract
A high performance liquid chromatography-inductively coupled plasma mass spectrometric(HPLC-ICP-MS) method was developed for the determination of 4 arsenic species,including arsenite(As(Ⅲ)),arsenate(As(V)),monomethylarsonic acid(MMA) and dimethylarsinic acid (DMA),in tobacco.The extract efficiencies of different extract solvents,extract solvent volume and extract time were investigated.The chromatographic conditions were also optimized.The milled tobacco samples were mechanically extracted with 15 mL mixture of methanol-water(1 ∶ 1) by ultrasonic method for 20 min at 30 ℃.The target species in tobacco were analyzed on an anion exchange cartridge (PRP-Xl00) using diammonium phosphate (pH 6.0) as mobile phase.Good linearities of 4 arsenic species were observed in the range of 0.1-25 μg/L with correlation coefficients not less than 0.997.The RSDs of intra-and inter-day were both not more than 4.2％.The limits of detection(LODs) were between 0.1 μg/L and 0.2 μg/L,and the limits of quantitation (LOQs) were between 0.2 μg/L and 0.5 μg/L.The spiked recoveries for all arsenic species were in the range of 83％-115％.Good stabilities were found with RSDs not more than 5.7％.The method was applied in the detection of 4 arsenic species in eight kinds of tobaccos,in which all arsenic species were detected and their contents from high to low were:As (V),MMA,As (Ⅲ) and DMA.The method was proved to be accurate,sensitive and convenient for analysis of arsenic species in tobacco sample.关键词
高效液相色谱-电感耦合等离子体质谱/砷形态分析/烟草Key words
high performance liquid chromatography-inductively coupled plasma-mass spectrometry(HPLC-ICP-MS) / arsenic species/ tobacco分类
化学化工引用本文复制引用
李莉,李伟青,申德省,崔维刚,储晓刚,张峰..高效液相色谱-电感耦合等离子体质谱法测定烟草中砷的形态[J].分析测试学报,2013,32(8):941-946,6.基金项目
质检公益性行业科研项目(201310134) （201310134）
中央级公益性科研院所基本科研业务费专项(2012JK031) （2012JK031）
