分析化学2013，Vol.41Issue(8)：1194-1198,5.DOI:10.3724/SP.J.1096.2013.30047
在线净化液相色谱串联质谱法测定动物源食品中金刚烷胺的残留
Determination of Amantadine Residues in Foods of Animal Origin by On-line Cleanup Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
A new method using online cleanup technology combined with tandem mass spectrometry (MS/MS) has been developed for determination of amantadine residues in foods of animal origin.The manual sample preparation was limited to extraction of simples by methanol-1％ trichloroactic acid (50∶ 50,V/V) or acetonitrile.The extract was online purified on cyclone MCX column where the sample matrix was washed away while the analytes were retained.Subsequently,the analytes were eluted from the extraction column onto the analytical column (Capcell Pak MG C18 column) by means of 5％ ammonia hydroxide methanol solution prior to chromatographic separation and MS/MS detection.The limit of quantization (LOQ) for milk was 0.25 μg/kg,and for animal tissues and egg was 0.5 μg/kg.The linearity is satisfactory with a correlation coefficient of ＞0.9995 at concentrations ranging from O.1 to 20 μg/L.Average recoveries of the analytes fortified at three levels were range from 83.3％ to 93.6％,and the relative standard deviations were range from 3.5％ to 5.9％.The method is suitable for quantitative analysis and confirmation of amantadine residues in foods of animal origin.关键词
在线净化液相色谱串联质谱/金刚烷胺/残留/动物源食品Key words
On-line cleanup liquid chromatography-tandem mass spectrometry/ Amantadine/ Residues/Foods of animal origin引用本文复制引用
艾连峰,马育松,陈瑞春,郭春海,康占省..在线净化液相色谱串联质谱法测定动物源食品中金刚烷胺的残留[J].分析化学,2013,41(8):1194-1198,5.基金项目
本文系国家质检总局科技计划项目(No.2013IK154)和河北检验检验局科研项目(No.HE2011K005)资助 （No.2013IK154）
