分析化学2013，Vol.41Issue(8)：1204-1208,5.DOI:10.3724/SP.J.1096.2013.20721
毛细管电泳法表征离子液体与蛋白质的相互作用
Interaction between Ionic Liquid and Protein Based on Capillary Electrophoresis
摘要
Abstract
The interaction between ionic liquid and protein was characterized by capillary zone electrophoresis (CZE).The effects of ionic liquid cationic head-group,alkyl side chain length and anion on interaction with bovine serum albumin were investigated,and the interactions between [C4mim] BF4 and myoglobin,bovine serum albumin,hemoglobin,bovine thrombin and transferrin were discussed.Based on affinity capillary electrophoresis (ACE),the binding constant Kb of four proteins binding with [C4mim] BF4 was 1.24 × 107,1.23×106,5.75 ×104 and 5.60× 104 L/mol,respectively.The results showed the interaction strength reduced in the tum of transferrin,hemoglobin,bovine serum albumin and myoglobin.The interaction between ionic liquid and protein could be characterized quantitatively and qualitatively by CZE and ACE.关键词
离子液体/蛋白质/相互作用/毛细管区带电泳/亲和毛细管电泳Key words
Ionic liquid/ Protein/ Interaction/ Capillary zone electrophoresis/ Affinity capillary electrophoresis引用本文复制引用
赵新颖,郭淑元,陈凡,屈锋,罗爱芹..毛细管电泳法表征离子液体与蛋白质的相互作用[J].分析化学,2013,41(8):1204-1208,5.基金项目
本文系国家重点基础研究发展计划(No.2012CB910603)和国家自然科学基金(No.21175011,20875009)资助项目 （No.2012CB910603）
