分析化学2013，Vol.41Issue(8)：1243-1248,6.DOI:10.3724/SP.J.1096.2013.21130
葡萄糖氧化酶在石墨烯-纳米氧化锌修饰玻碳电极上的直接电化学及对葡萄糖的生物传感
Direct Electrochemistry of Glucose Oxidase on Graphene-ZnO Nanocomposite Modified Glassy Electrode and Its Application for Glucose Biosensing
陈慧娟 1朱建君 1余萌1
作者信息
- 1. 信阳师范学院化学化工学院,信阳464000
- 折叠
摘要
Abstract
Graphene was coated on glassy carbon electrode and ZnO was then electrodeposited on the modified electrode.The biosensor was fabricated for sensitive determination of glucose after glucose oxidase was immobilized on the modified electrode.Scanning electron microscope was used to characterize the nano-composite film.The electrochemical behaviors of glucose oxidase on the modified electrode were investigated in the range of-0.7 V to-0.1 V by cyclic voltammetry.The experimental results demonstrated that the nanocomposite well retained the activity of glucose oxidase and the biosensor exhibited excellent electrocatalytic activity toward the redox of glucose.In 0.1 mol/L PBS (pH 7.0),the glucose oxidase adsorbed onto the graphene/ZnO composite film exhibits a pair of well-defined nearly reversible redox peaks and fine catalysis to the oxidation of glucose.The electron transfer rate constant (ks) of glucose oxidase at the modified electrode was 1.42 s-1,and Michaelis-Menten constant (Kampp) was 14.2 μmol/L.A good linear relationship was obtained in the range of 2.5 × 10-6-1.5 × 10-3 mol/L,with the limits of detection of 2.4 × 10-7 mol/L (S/N =3).The biosensor has good conductivity,stability and repeatability,and it can be used to analyze real samples.关键词
石墨烯/氧化锌/葡萄糖氧化酶/直接电化学/生物传感器Key words
Graphene / Zinc oxide / Glucose oxidase / Direct electrochemistry / Biosensor引用本文复制引用
陈慧娟,朱建君,余萌..葡萄糖氧化酶在石墨烯-纳米氧化锌修饰玻碳电极上的直接电化学及对葡萄糖的生物传感[J].分析化学,2013,41(8):1243-1248,6.
