感染、炎症、修复2013，Vol.14Issue(2)：84-87,4.DOI:10.3969/j.issn.1672-8521.2013.02.007
丙酮酸腹膜透析液对大鼠失血性休克静脉液体复苏后腹腔脏器的保护作用
Protective effects of intraperitoneal administration of pyruvate in peritoneal dialysis fluid on abdominal organ function in rats subjected to fluid resuscitation following hemorrhagic shock
摘要
Abstract
Objective:To investigate the protective effects of intraperitoneal administration of pyruvate in peritoneal dialysis fluid on abdominal organ function in rats subjected to fluid resuscitation following hemorrhagic shock (HS).Methods:Forty Sprague-Dawley (SD) rats were randomly divided into 4 groups:intravenous resuscitation group (group VR),intraperitoneal normal saline group (group DPR),intraperitoneal sodium lactate dialysis fluid group (group L),and intraperitoneal pyruvic acid sodium dialysis fluid group (group P).45％ of whole body blood was withdrawn via femoral artery in 4 groups of rat.In group VR,rats were resuscitated with the shed blood and sodium lactate fluid of 2 times the volume of blood loss 1 hour later.In groups DPR,L and P,after resuscitation with the shed blood and sodium lactate fluid,the rats received intraperitoneal administration of 20 ml normal saline,20 ml sodium lactate dialysis fluid or 20 ml pyruvic acid sodium dialysis fluid,respectively,for a duration of 30 minutes.The right common carotid artery was cannulated to monitor mean arterial pressure (MAP)with Picco cardiac function monitor.Blood flow volume of intra-abdominal organ was monitored by Laser Doppler Velocimetry at 180 minutes and 360 minutes after fluid resuscitation.180 minutes and 360 minutes after shock,rats were sarcrificed by femoral artery blood letting.Water content of organs was measured by proportion of dry and wet method.The activities of alanine aminotransferase (ALT),creatinine (Cr),and diamine oxidase (DAO)were measured by biochemistry.Results:MAP was maintained at (35 ±5) mmHg after hemorrhagic shock.The difference was not significant among 4 groups 60 minutes after shock (P＞0.05).After peritoneal resuscitation,MAP in L and P groups was obviously elevated compared with group VR (P＜0.05),while ALT,Cr and DAO were decreased,blood flow of abdominal organ was increased,and visceral tissue edema was alleviated (P＜0.05 or P＜0.01).These changes were more significant in group P 360 minutes after hemorrhagic shock.Conclusions:The results indicate that intraperitoneal resuscitation with sodium pyruvate fluid has a protective effect on abdominal organ function in rats subjected to fluid resuscitation following hemorrhagic shock.关键词
丙酮酸/休克,失血性/腹腔复苏/腹腔脏器Key words
Pyruvate / Hemorrhagic shock Intraperitoneal / resuscitation / Inner organs引用本文复制引用
马丽,林志龙,罗红敏,杜明华,王欢,白晓东,周方强,胡森..丙酮酸腹膜透析液对大鼠失血性休克静脉液体复苏后腹腔脏器的保护作用[J].感染、炎症、修复,2013,14(2):84-87,4.基金项目
国家重点基础研究发展(973)计划(2012CB518101) （973）
