国际妇产科学杂志2013，Vol.40Issue(4)：372-374,385,4.
高效液相色谱法测定卵巢癌患者血浆中紫杉醇浓度的研究
Determination of Paclitaxel in Ovarian Neoplasm Patients' Plasma by HPLC
摘要
Abstract
Objective:An HPLC method was established for determination of paclitaxel's concentration in ovarian neoplasm patients' plasma.Methods:Each test 500 μL plasma sample was alkalinized by sodium bicarbonate firstly,and then extracted by diethyl ether.Docetaxel was used as the internal standard.Analytical column was Ultimate Acclaim(R) 120 C18 (5 μm,4.6 mm×250 mm).The isocratic mobile phase was the acetonitrile and 0.1％ phosphoric acid with the rate of 55∶45 (V/V).The flow rate was 1.3 mL/min,the temperature of chromatographic column was 20 ℃ and the detective UV wavelength was 227 nm.Results:The calibration curve was linear over the concentration range of 0.1-10.0 mg/L (r=0.9989).The limit of detection was 0.04 mg/L (S/N=3),the relative recovery was (98.5±4.1)％ and the RSD of intra-day and inter-day were lower than 10％ respectively.Conclusions:The method is simple,rapid and repeatable.It is suitable for chemotherapeutic drug monitoring and the pharmacokinetics studying in ovarian neoplasm patients.关键词
卵巢肿瘤/紫杉酚/色谱法,高压液相/血药浓度Key words
Ovarian neoplasms/ Paclitaxel / Chromatography, high pressure liquid/ Plasma concentration引用本文复制引用
董滨华,孙蓬明,宋一一,曾凡湘,林芬,毛晓丹..高效液相色谱法测定卵巢癌患者血浆中紫杉醇浓度的研究[J].国际妇产科学杂志,2013,40(4):372-374,385,4.基金项目
福建省科技厅社会发展重点资助项目(2009Y0007) （2009Y0007）
