华东师范大学学报（自然科学版）Issue(5)：71-79,9.DOI:10.3969/j.issn.1000-5641.2013.05.009
漂浮绿藻中缘管浒苔(Ulva linza)鉴定方法的探索
An exploratory study on identification means of Ulva linza from floating green algae
摘要
Abstract
To study on morphological characteristics of Ulva linza from floating green algae,we selected specimens with the typical features of U.linza.Only 4 specimens from 25 samples were determined as U.linza from the morphological characteristics results.The molecular biology research by means of nuclear-encoded ITS sequences analysis was also carried out.Fifteen floating specimens with characters of U.linza clustered into the LPP (U.linza-procera-prolifera) complex group including the attached species DF05 from Jiangsu Province,QD03 from Shandong Province and XS01,XS02 from Zhejiang Province.The floating green algae from Rudong inland river and coastal pool of Sheyang were included in LPP complex as well.U.flexuosa group included two specimens that just were fixation growth algae from Fujian Province.U.compressa group had four specimens,and they all were floating green algae.A great disparity existed between the morphological characteristics and the molecular results.Identification of U.linza and other floating green algae based on either morphology or ITS sequence unilaterally was not reliable.Almost all of floating green algae from the same location had cgrtain morphological types,and they were considered to be different species by the ITS sequences analysis.The result of N-J tree and ITS data showed that the species in LPP complex and U.compressa existed in the green tide.But U.flexuosa did not exist in the floating green algae in this research.关键词
绿潮/缘管浒苔/形态学/ITS/分子生物学Key words
green tide/ Ulva linza/ morphology/ ITS/ molecular biology分类
生物科学引用本文复制引用
陈斌斌,马家海,SHIN Jong-Ahm,高嵩..漂浮绿藻中缘管浒苔(Ulva linza)鉴定方法的探索[J].华东师范大学学报（自然科学版）,2013,(5):71-79,9.基金项目
国家海洋局公益项目(201205010) （201205010）
国家海洋局重点实验室开放研究基金(MATHAB00000000) （MATHAB00000000）
科技部国家科技支撑计划(2012BAC07B03) （2012BAC07B03）
