华东师范大学学报（自然科学版）Issue(5)：96-101,6.DOI:10.3969/j.issn.1000-5641.2013.05.012
维生素B12的毛细管电泳—化学发光检测研究
Investigation on chemiluminescence detection of vitamin with capillary electrophoresis
摘要
Abstract
Based on the chemiluminescence (CL) catalysis of vitamin B12 (VB12) in luminol-H2O2 system,a novel capillary electrophoresis-chemiluminescence (CE-CL) method was developed for the accurate determination of this important nutrient substance.First of all,sodium hydrosulfite (Na2S2O4) was found to be used as an competent reduction reagent to pre-reduce VB12(Co(Ⅲ)) into VB12(Co(Ⅱ)),which produced strong CL emission in the CL reaction of luminol-H2O2.In this process,some interfering CL noise was observed and capillary electrophoresis (CE) was taken to remove it by efficient separation.In this way,VB~ could be reduced gently and determined accurately by the catalyzed CL signal with our lab-constructed CE-CL system.The proposed CE-CL determination could be finished in 20 min with a detection limit (LOD) of 2×10-7 mol/L (S/N=3),a linear range from 6×10-7 to 6×10-4 mol/L (r3=0.968) and a relative standard deviation (RSD)of 2.4％ (n=9).Utilizing this method,satisfactory results were obtained by testing VB12 tablets with a recovery of 97％～106％.Compared to previously reported CL analysis of VB13,we dent with the interference components in pre-reducing solution for the first time to gain more reliable data with convenient operations.关键词
维生素B12/化学发光/毛细管电泳/连二亚硫酸钠Key words
vitamin B12/ chemiluminescence/ capillary electrophoresis/ sodium hydrosulfite分类
化学化工引用本文复制引用
朱金坤,舒露,吴敏,王清江,何品刚,方禹之..维生素B12的毛细管电泳—化学发光检测研究[J].华东师范大学学报（自然科学版）,2013,(5):96-101,6.基金项目
新世纪优秀人才支持计划(NCET-08-0191) （NCET-08-0191）
安徽省教育厅自然科学研究项目(KJ2012B059) （KJ2012B059）
This work was financially supported by program for New Century Excellent Talents in University (NCET-08-0191) and Natural Science Fund of Anhui Education Department (KJ2012B059). （NCET-08-0191）
