河南农业科学2013，Vol.42Issue(8)：67-70,4.
黄瓜绿斑驳花叶病毒抗原表位预测
Prediction of Epitopes in Coat Protein of Cucumber Green Mottle Mosaic Virus
摘要
Abstract
In the study,the epitopes in coat protein of cucumber green mottle mosaic virus (CGMMV) were predicted by several algorithms in some softwares.It was in favor of producing antiserum of CGMMV on the basis of the epitopes technique.Based on the nucleotide sequences of coat protein (CP) gene of different CGMMV isolates,phylogenetie analysis with the neighbor-joining algorithm of MEGA software showed that all isolates of CGMMV fell into 2 clades.The clades were not related with the geological origins and hosts of CGMMV.Some characteristics of CP,such as antigenic index and protein secondary structure,were analyzed with the algorithms in different softwares.Combined with predicted tertiary structure,the predicted epitopes were located in CP of amino acid position 3-15,80-90,and 90-100,respectively.关键词
黄瓜绿斑驳花叶病毒/抗原表位/系统进化分析/蛋白结构Key words
cucumber green mottle mosaic virus/ epitopes/ phylogenetic analysis/ protein structure分类
农业科技引用本文复制引用
杨洪一,孙立娜,张杰,任静..黄瓜绿斑驳花叶病毒抗原表位预测[J].河南农业科学,2013,42(8):67-70,4.基金项目
黑龙江省教育厅科研项目(11541033) （11541033）
