河南农业科学2013，Vol.42Issue(8)：79-81,85,4.
宁夏固原地区马铃薯M病毒的检测鉴定
Detection and Identification of Potato Virus M in Guyuan of Ningxia
摘要
Abstract
In order to know the occurrence of potato virus M (PVM) in Guyuan of Ningxia,18 samples displaying symptoms of leaf distortion,stem necrosis,and plant dwarf were collected and tested.Enzyme-linked immunosorbent assay (ELISA) showed positive reaction of PVM in two samples (No.5 and No.10).The two samples were detected by RT-PCR and a target band with size about 416 bp was amplified from both samples.The sequence analysis of amplified fragment showed that the highest homology of the two sequences with other isolates of PVM was 99％ and 98％,respectively,which further indicated that the samples were infected by PVM.It was the first report of PVM occurrence in Guyuan of Ninxia,which provided a basis for prevention and control of potato virus disease in this region in the future.关键词
宁夏固原/马铃薯M病毒/检测/鉴定Key words
Guyuan of Ningxia/ potato virus M/ detection/ identification分类
农业科技引用本文复制引用
张永江,刘忠梅,燕照玲,刘洪义,马洁,陈林,辛言言,马云霞..宁夏固原地区马铃薯M病毒的检测鉴定[J].河南农业科学,2013,42(8):79-81,85,4.基金项目
质检公益性行业科研专项(201110035) （201110035）
出入境检验检疫行业标准项目(2012B184) （2012B184）
