河南农业科学2013，Vol.42Issue(8)：146-149,4.
利用固定化木聚糖酶分离小麦木聚糖酶抑制蛋白的研究
Separation of Xylanase Inhibitor Protein from Wheat Seeds Using Immobilised Xylanase
摘要
Abstract
Based on the special interaction between xylanase and xylanase inhibitor protein,inhibitor protein was purified from the protein crude extract of wheat seeds using affinity chromatography with immobilized xylanse under the conditions of different pH values,temperatures and times.Subsequently,the desorption experiment of enzyme-inhibitor complex was carried out under different pH values.The results showed that the optimum adsorption pH value was 6.5,temperature was 30 ℃,time was 50 min; the optimum desorption pH value was 8.5.Purification of inhibitor protein using affinity chromatography with immobilized xylanse is a better new method.关键词
木聚糖酶/木聚糖酶抑制蛋白/小麦/亲和层析Key words
xylanase/ xylanase inhibitor protein/ wheat/ affinity chromatography分类
农业科技引用本文复制引用
刘新育,张贝贝,李学琴,陈红歌..利用固定化木聚糖酶分离小麦木聚糖酶抑制蛋白的研究[J].河南农业科学,2013,42(8):146-149,4.基金项目
河南省教育厅科学技术研究重点项目(12A180013) （12A180013）
