家畜生态学报2013，Vol.34Issue(6)：36-39,4.
不同浓度玻璃化冷冻液对牛未成熟卵母细胞发育能力的影响
Effect of Different Vitrification Solutions on the Development Competent of Immature Bovine Oocytes
摘要
Abstract
The aim of this study was to evaluate the effect of different vitrification solutions on the potential development competent of immature oocytes in bovine.In experiment 1,cumulus oocyte complexes (COCs) were placed in vitrification solution 1 (VS1,10％ EG+ 10％ DMSO) or vitrification solution 2 (VS2,20％ EG+20％ DMSO) or vitrification solution 3(VS3,25 EG+25％ DMSO) for either 30 or 60 s.There were no differences among VS1,VS2 and control group for maturation rates(P＞0.05).However,maturation rate of 60 s in VS3 was decreased than that of the control group(P＜0.01).In experiment 2,COCs were cryopreseved in open pulled straw (OPS) in different solutions of VS1 or VS2 or VS3.The highest blastocyst rate (5.4％) was in VS2,the lowest was in VS1 (0),there were no defferences between VS2 and VS3 (3.6％) groups.It can be concluded that solution contained 20％ EG and 20％ DMSO can be used for vitrification of immature bovine oocytes,and the exposing time of COCs in the cryoprotectant should be limited in 30 s.关键词
牛/卵母细胞/玻璃化冷冻液Key words
bovine/ Oocyte / vitrification solution分类
农业科技引用本文复制引用
栗颖华,禹学礼,张德勋,陈君怡,许亚坤,李晓霞,昝林森..不同浓度玻璃化冷冻液对牛未成熟卵母细胞发育能力的影响[J].家畜生态学报,2013,34(6):36-39,4.基金项目
国家自然科学基金(31172207) （31172207）
国家科技支撑计划(2006BAD04A11,2011BAD28B04-03,2012BAD12B07) （2006BAD04A11,2011BAD28B04-03,2012BAD12B07）
