家畜生态学报2013，Vol.34Issue(6)：62-65,4.
兔出血症病毒ZB株的分离与鉴定
Isolation and Identification of Virus ZB Strain from Rabbit Hemorrhagic Disease
杨丽梅 1马力 2王艳萍 1沈志强 2李峰 1郭时金 2张颖 1张志美 2徐倩倩1
作者信息
- 1. 山东省滨州畜牧兽医研究院,山东滨州256600
- 2. 山东绿都安特动物药业有限公司,山东滨州256600
- 折叠
摘要
Abstract
In order to determine the cause of death and analyze its pathogen in the rabbit farm,the study conducted clinical observation,pathological examination,virus isolation,purity test,hemagglutination test,pathogenicity test,specificity test,immunity test,RT-PCR and sequencing.One strain of rabbit hemorrhagic disease virus was successfully isolated by the mortality rabbit disease material inoculated into the healthy susceptible rabbits.The isolated virus has no bacteria,mold,mycoplasma; the isolated virus hemagglutination titer is 1:1024 on human "O" red cell; the isolated virus can cause deaths of susceptible rabbits in 96 h after inoculated virus; rabbit hemorrhagic disease virus positive serum has neutralizing effect on the isolated virus; the preparation of inactivated vaccine with the isolated virus has good immunogenicity.RT-PCR sequencing proved the isolated virus was RHDV.This study successfully isolated one strain of rabbit hemorrhagic disease virus,which provides a reference for molecular epidemiology investigation of RHDV.关键词
兔出血症病毒/分离/鉴定Key words
rabbit haemorrhagic disease virus/ isolation/ identification分类
农业科技引用本文复制引用
杨丽梅,马力,王艳萍,沈志强,李峰,郭时金,张颖,张志美,徐倩倩..兔出血症病毒ZB株的分离与鉴定[J].家畜生态学报,2013,34(6):62-65,4.
