基础医学与临床2013，Vol.33Issue(9)：1107-1111,5.
Fzr1基因沉默促进滋养层细胞的侵袭与增殖
Promotion of the invasion and proliferation of trophoblast cells by silencing Fzr1
摘要
Abstract
Objective To explore the spatial and temporal expression of Fzrl protein during the development of placenta,and observe the effect of Fzr1 on the invasion and proliferation of trophoblast cells.Methods To observe the expression level and localization of Fzr1 protein using Western blot and immunohistochemical methods,and to detect the effect of Fzr1 on trophoblast cell invasion and proliferation by RNA interference technology,Transwell and MTS methods.Results 1) Fzrl protein was significantly expressed in the cytotrophoblast and syncytiotrophoblast cells of villi during early pregnancy.Positive signals were mainly located in the cytoplasm.In the third trimester of pregnancy,Fzr1 protein was expressed in the syncytiotrophoblast,and expression level significantly decreased.Positive signal was located in the nucleus.Western blot studies showed that Fzr1 protein levels in the third trimester decreased by 4.88 times as compared with early pregnancy (P ＜ 0.01).2) Fzrl siRNA significantly enhanced the invasion of JAR cells compared with blank control (P ＜ 0.01),but scrambled siRNA had no significantly change.3)Fzr1 siRNA significantly enhanced the proliferation of JAR cells compared with blank control (P ＜ 0.01),but scrambled siRNA had no significantly change.Conclusions Fzr1 protein is temporally and spatially expressed at the placenta of normal pregnancy,and Fzr1 gene silencing may promote the invasion and proliferation of trophoblast cell.关键词
Fzr1/RNA干扰/滋养层细胞/侵袭/增殖Key words
Fzr1/ siRNA/ trophoblast cells/ invasion/ proliferation分类
医药卫生引用本文复制引用
张保平,钟婷,张莉,况海斌..Fzr1基因沉默促进滋养层细胞的侵袭与增殖[J].基础医学与临床,2013,33(9):1107-1111,5.基金项目
国家自然科学基金(30960118,31260248) （30960118,31260248）
江西省教育厅基金(GJJ12071) （GJJ12071）
