基础医学与临床2013，Vol.33Issue(9)：1123-1128,6.
丹参酮ⅡA通过诱导C/EBPβ表达促进人源NB4和MR2细胞系分化
Tan Ⅱ A triggers the differentiation of NB4 and MR2 cells by up-regulated expression of C/EBPβ
摘要
Abstract
Objective To Investgiate the expression of C/EBPβ in APL cells during differentiation induced by Tan Ⅱ A.Methods Tan Ⅱ A treated NB4 and MR2 cells 120h,the characterizations of differentiation were examined by morphology and membrane differentiation antigens;The mRNA and protein expressions of C/EBPβ in different concentrations of Tan Ⅱ A-intervened APL cells were examined by RT-PCR and Western blot ; APL cells were treated with different concentrations of Tan Ⅱ A,the characterizations of differentiation were examined by membrane differentiation antigens.Results Tan Ⅱ A induced the differentiation of ATRA-sensitive and ATRA-resistant APL cells (P ＜ 0.05) ; Tan Ⅱ A dose-dependend upregulated the expression of C/EBPβ in mRNA and protein (P ＜ 0.05) ; Tan Ⅱ A promoted the differentiation of the NB4 and MR2 cells in a dose-independent manner and exerted its strongest effect at a concentration of 1 mg/L.Conclusions Tan Ⅱ A promotes the differentiation of ATRA-sensitive and ATRA-resistant APL cells and exertes its strongest effect at a concentration of 1 mg/L; C/EBPβ played a key role in Tan Ⅱ A-induced differentiation.关键词
丹参酮ⅡA/C/EBPβ/分化Key words
Tan Ⅱ A/ C/EBPβ / differentiation分类
医药卫生引用本文复制引用
张开基,王季石,李健,曹金竹,羊裔明..丹参酮ⅡA通过诱导C/EBPβ表达促进人源NB4和MR2细胞系分化[J].基础医学与临床,2013,33(9):1123-1128,6.基金项目
国家自然科学基金(30470748) （30470748）
