基础医学与临床2013，Vol.33Issue(9)：1141-1145,5.
bFGF2抑制BMP9诱导小鼠间充质干细胞C3H10T1/2向成骨细胞分化
bFGF-2 inhibits bone morphogenetic protein9-induced osteogenic differentiation of C3H10T1/2 mesenchymal stem cells
摘要
Abstract
Objective Observe the effect of FGF2 in BMP9-induced osteogenic differentiation of C3H10T1/2 mesenchymal stem cells,and investigate its mechanism.Methods 3H10T1/2 cells were infected by adenovirus recombined with FGF2 and BMP9,then the early osteogenic maker alkaline phosphatase(ALP) activity was detected by quantitative and staining assay,later osteogenic maker calcium deposition was determined by Alizarin Red S staining,pl2SBEluc activity was detected by quantitative,Smad1/5/8,Runx2 and OCN were determined by Western blot.Results BMP9-induced early osteogenic marker ALP,and late osteogenic markers matrix mineralization and osteocalcin (OCN) was inhibited by FGF2,BMP-Smad1/5/8 pathway and Runx2 were inhibited by FGF2.Conclusions FGF2 inhibits bone morphogenetic protein9-induced osteogenic differentiation of C3H10T1/2 mesenchymal stem cells.关键词
碱性成纤维细胞生长因子/骨形态发生蛋白9/间充质干细胞/成骨分化Key words
FGF2/ BMP9/ mesenchymal stem cells / osteogenic differentiation分类
生物科学引用本文复制引用
宋涛,赵艳芳,刘跃亮,徐静,王文娟,罗进勇..bFGF2抑制BMP9诱导小鼠间充质干细胞C3H10T1/2向成骨细胞分化[J].基础医学与临床,2013,33(9):1141-1145,5.基金项目
国家自然科学基金(31071304,30800658) （31071304,30800658）
