基础医学与临床2013，Vol.33Issue(9)：1155-1159,5.
Wnt/β-catenin信号途径抑制Raw264.7分化
Wnt/β-catenin signaling pathway in inhibition of Raw264.7 cell differentiation
摘要
Abstract
Objective To learn the regulation of Wnt/β-catenin signaling pathway in the mouse monocyte/macrophage Raw264.7 differentiation and maturation.Methods The cells were divided into four groups:the negative control group (empty Raw264.7),the experimental control group (infected with Ad-GFP),the positive control group (50 ng/mL RANKL induced) and the experimental group (infected with Ad-β-catenin).RNA were extracted after the cells was treated for 3,6,9 days respectively,The expression of nuclear factor KB receptor,Tartrateresistant acid phosphatase,Cathepsin K and Metalloproteinase-9 gene was detected by fluorescence quantitative RTPCR respectively.Cells treated for 6 days were used to observe the differentiation of Raw264.7 by Tartaric acid-resistant phosphatase (TRAP) staining and expressions of TRAP and Cathepsin K protein by Western blot.Results qRT-PCR showed that Ad-β-catenin group decreased the mRNA expression of RANK,TRAP,Cathepsin K and MMP-9 gene.TRAP staining showed that 50 ng/mL RANKL successfully induced Raw264.7 into multi-cores.Few multinucleated cells in the control group and the Ad-GFP group were found,In Ad-β-catenin group cells were mononuclear.The TRAP and Cathepsin K protein expression was significantly reduced shown by Western blot (P ＜ 0.05).Conclusion Wnt/β-catenin signaling pathway can inhibit Raw264.7 differentiate into mature osteoclasts.关键词
Wnt/β-catenin/Raw264.7/RANKL/TRAPKey words
Wnt/β-catenin/ Raw264.7/ RANKL/ TRAP分类
医药卫生引用本文复制引用
李红丽,芦永良,申利红,冯涛,黄佳袆..Wnt/β-catenin信号途径抑制Raw264.7分化[J].基础医学与临床,2013,33(9):1155-1159,5.基金项目
重庆市自然科学基金(CSTC,2010BB5100) （CSTC,2010BB5100）
