解放军医学杂志2013，Vol.38Issue(7)：548-551,4.
促黄体激素释放激素a(LHRHa)靶向鸦胆子油脂质体的制备及质量评价
Preparation and quality evaluation of LHRHa-targeted Brucea javanica oil liposomes
摘要
Abstract
Objective To prepare luteinizing hormone-releasing hormone a (LHRHa) targeted Brucea javanica liposomes and evaluate its quality.Methods The LHRHa-targeted Brucea javanica liposome was prepared by thin layer dispersion together with biotin-streptavidin bridge method.The optimum formation was selected by means of orthogonal design of experiment.The morphology of liposome was observed with transmission electron microscope.Zetasizer Nano ZS analyzer was used to measure the particle size and zeta potential.The entrapment efficiency was determined by ultra-violet spectroscopy and column chromatography.Centrifugal acceleration experiment and determination of leak rate were performed to prove the liposome stability.The targeting ability ofliposome was appraised by cell experiment in vitro.Results The formed optimum formula was as follows:the ratio of lecithin to cholesterol was 4∶1,Brucea javanica oil∶lipid was 3∶10,DSPE-PEG (2000)-Biotin:lecithin content was 3％,ultrasonic-homogenized for 8 minutes.Liposomes were round in shape,the average diameter and zeta potential of liposome were 155.1 ± 14.5mm and-(24.1 ± 0.54) mV,respectively.The average entrapment efficiency was 92.2％.Binding capacity with the A2780/DDP cell line in the LHRHa-targeted liposomes was 2.7 times higher than that in the non-targeting liposomes.Conclusion The technique of preparing LHRHa-targeted Brucea javanica liposome is suitable,and high in entrapment efficiency,with good stability and targeting ability.关键词
鸦胆子油脂质体/促黄体激素/卵巢肿瘤Key words
Brucea javanica liposomes/ luteinizing hormone/ ovarian neoplasms分类
医药卫生引用本文复制引用
刘晓娟,常淑芳,孙江川,叶红霞,曾飚,朱轶,朱深银,王驰..促黄体激素释放激素a(LHRHa)靶向鸦胆子油脂质体的制备及质量评价[J].解放军医学杂志,2013,38(7):548-551,4.基金项目
国家自然科学基金(30801228) （30801228）
重庆市卫生局重点项目(2010-1-39) （2010-1-39）
重庆市中医处重点项目(2010-1-6) （2010-1-6）
This work was supported by the National Natural Science Foundation of China (30801228),the Key Item of Chongqing Health Bureau (2010-1-39),and the Key Project of Chongqing Traditional Chinese Medicine (2010-1-6) （30801228）
