激光生物学报2013，Vol.22Issue(2)：103-113,11.DOI:10.3969/j.issn.1007-7146.2013.02.002
受激发射损耗显微术(STED)的机理及进展研究
A Review on Concept and Development of Stimulated Emission Depletion Microscopy(STED)
摘要
Abstract
Due to the diffraction of the optical elements,the resolution of conventional optical microscopy has long been limited to about half the illumination wavelength.By introducing a depletion beam to inhibit the potential fluorescence through stimulated emission,STED microscopy is able to achieve a spatial resolution beyond the diffraction limit.Since being proposed,STED microscopy has been improved in many aspects and widely be applied in the biomedical and material researches to perform multi-functional imaging.This paper elaborates the concepts and key techniques in STED microscopy,reviews its development and latest progresses and also introduces its wide application in scientific researches.关键词
光学显微/荧光/受激发射/超分辨Key words
optical microscopy/fluorescence/stimulated emission/super-resolution分类
数理科学引用本文复制引用
李帅,匡翠方,丁志华,郝翔,顾兆泰,葛剑虹,刘旭..受激发射损耗显微术(STED)的机理及进展研究[J].激光生物学报,2013,22(2):103-113,11.基金项目
国家自然科学基金(61205160) （61205160）
浙江省钱江人才计划基金(2011R10010) （2011R10010）
教育部博士点基金(20110101120061,20120101130006) （20110101120061,20120101130006）
