激光生物学报2013，Vol.22Issue(2)：154-158,5.DOI:10.3969/j.issn.1007-7146.2013.02.010
抗果蝇MRJ蛋白单克隆抗体的制备和鉴定
Preparation and Identification of Anti-Drosophila MRJ Protein Monoclonal Antibody
摘要
Abstract
Preparation of anti-Drosophila MRJ protein monoclonal antibody could be used for research of mrj biological function in Drosophila.The recombinant plasmid pET28a-mrj in Rosetta was induced with IPTG to express MRJ recombinant protein.The recombinant protein purified by Ni-IDA gel column was used to immunized BALB/c mice.Then the spleen cells of immunized mice and mouse myeloma cells SP2/0 were used for cell fusion.A hybridoma cell line which secrete anti-mrj monoclonal antibodies was obtained through subcloning and screening progress.The monoclonal antibodies was identified by Elisa and Western Blot.The results show that the anti-mrj monoclonal antibodies can binding to MRJ protein of prokaryotic and eukaryotic cells specificity.It could be used for biology function research of mrj gene.关键词
mrj/细胞融合/单克隆抗体Key words
mrj/cell fusion/monoclonal antibody分类
生物科学引用本文复制引用
廖四芳,刘丹,宋文,刘明,赵阳,唐超,吴秀山,王跃群..抗果蝇MRJ蛋白单克隆抗体的制备和鉴定[J].激光生物学报,2013,22(2):154-158,5.基金项目
国家自然科学基金资助项目(30930054,31071999,31272396) （30930054,31071999,31272396）
