解剖学杂志2013，Vol.36Issue(4)：705-710,6.DOI:10.3969/j.issn.1001-1633.2013.04.001
原发性高血压进程中下丘脑内神经元型一氧化氮合酶和血管紧张素Ⅱ1型受体的共表达
Coexpression of neuronal NO synthase and angiotensin Ⅱ type 1 receptor in the hypothalamus in hypertensive condition
摘要
Abstract
Objective:To investigate the colocalization and expression of neuronal NO synthase (nNOS) with Angiotensin Ⅱ 1 type receptor (AT-1R) as well as arginine vasopressin (AVP) and nNOS mRNA in the hypothalamic nuclei of Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR).Methods:The dual immunofluorescence staining combined with RTPCR technology were used.Results:We found there were a quantity of nNOS and AT-1R immunoreactive neurons in the paraventricular nucleus (PVN) and supraopticnucleus (SON).The ratios of coexistence varied in the hypothalamus of SHR and WKY rats,and that of SHR was higher than WKY rats.We found that the nNOS mRNA and AVP mRNA levels were up-regulated in the SHR group.Conclusion:Nitric oxide (NO) might act as an inhibitory regulator of the activity of RAS in response to hypertension,but the repression cannot reverse the over-activity of Angiotensin Ⅱ and AVP.关键词
高血压/神经元型一氧化氮合酶/血管紧张素Ⅱ1型受体/加压素/下丘脑Key words
hypertension/ neuronal nitric oxide synthase/ angiotensin Ⅱ type 1 receptor/ arginine vasopressin/ hypothalamus引用本文复制引用
张露青,张枫,李雷,丁炯..原发性高血压进程中下丘脑内神经元型一氧化氮合酶和血管紧张素Ⅱ1型受体的共表达[J].解剖学杂志,2013,36(4):705-710,6.基金项目
国家自然科学基金(81200184) （81200184）
江苏省高校自然科学研究计划项目(11KJB310004) （11KJB310004）
