解剖学杂志2013，Vol.36Issue(4)：724-728,封2,6.DOI:10.3969/j.issn.1001-1633.2013.04.005
核因子-κB激活与细胞凋亡在重症急性胰腺炎肺损伤中的作用
Role of nuclear factor-κB activation and cell apoptosis in severe pancreatitis-associated lung injury
摘要
Abstract
Objective:To explore the relationship between intrapulmonary activition of nuclear factor-κB (NF-κB),cell apoptosis and pulmonary injury in rats with severe acute pancreatitis (SAP).Methods:SD rats were randomly divided into control group,SAP group and PDTC pretreated group.SAP was induced by retrograde injection of 5％ sodium taurocholate into the bile-pancreatic duct (0.1 ml/100 g).Histological changes of the lungs were observed after hematoxylin-eosin (HE) staining.Activation of NF-κB in the pulmonary tissues was detected by immunohistochemical methods.The cell apoptosis in the pulmonary tissues was observed by using TUNEL method.Results:A little positive signals were observed and a small number of apoptosis cells were seen in the epithelial and interstitial cells of the lung alveolus in the control group.The structure of cells was basically normal.In type Ⅱ alveolar epithelial cells and vascular endothelial cells,mitochondria swelling appeared in all SAP groups,the basement membrane widened,the nuclear chromatin aggregated to border,interstitial edema and the electron density increased.The expression of NF-κB and apoptosis index significantly increased and exhibited changes,and reached its peak at 6h.The positive signals and apoptotic cells were mostly observed in the neutrophils,macrophages,bronchial epithelial cells,alveolar epthelial cells and partial microvascular endothelium cells.The PDTC pretreated groups showed a decrease in pathological changes.The expression levels of NF-κB and apoptosis index were significantly weaker than those in the SAP groups,but still significantly higher than those in the control group.Conclusion:The activation of NF-κB may be involved in the SAP lung injury through inducing apoptosis.PDTC might inhibit the activation of NF-κB and apoptosis and then effectively alleviate severity of lung injury.关键词
核因子-κB/细胞凋亡/重症急性胰腺炎/肺损伤/大鼠Key words
nuclear factor-κB/ apoptosis/ severe acute pancreatitis/ lung injury/ rat引用本文复制引用
阚氏海,于振海,王志强,孔凡镇,唐菁,金昌洙..核因子-κB激活与细胞凋亡在重症急性胰腺炎肺损伤中的作用[J].解剖学杂志,2013,36(4):724-728,封2,6.基金项目
山东省自然科学基金(ZR2011CQ041) （ZR2011CQ041）
山东省高等学校科技计划项目(J08LJ53) （J08LJ53）
