解剖学杂志2013，Vol.36Issue(4)：732-735,767,5.DOI:10.3969/j.issn.1001-1633.2013.04.007
急性染镉对大鼠心肌细胞闰盘超微结构和连接蛋白43表达的影响及黄芪甲苷的拮抗作用
Astragaloside Ⅳ antagonizes effects of cadmium on cardiomyocyte intercalated disc ultrastructure and expression of connexin 43 in rat
姚志勇 1廖晓岗 1宁巍 1王红 1范京川 1李兆飞1
作者信息
- 1. 重庆医科大学生命科学研究院,重庆400016
- 折叠
摘要
Abstract
Objective:To observe the effect of cadmium (Cd) on cardiomyocyte intercalated disc ultrastructure and expression of connexin 43 (Cx43) in rats,and the protection mechanism of astragaloside Ⅳ (As Ⅳ) on it.Methods:SD rats were randomly divided into 3 groups:control group,Cd group (rats were treated with Cd 1 mg · d-1 · kg-1 body weight by intraperitoneal injection),Cd+As Ⅳ group (the dose of cadmium was 1 mg · d-1 · kg-1 body weight,the dose of As Ⅳ respectively were 5 mg· d-1 · kg.-1 body weight and 10 mg · d-1 · kg-1 body weight).The rats after 1-week or 2-week treatment were anesthetized for thoracotomy,the ventricular tissues were respectively adopted for light and electron microscope observation and irnrnunohistochemistry.Results:The expression of Cx43 was significantly reduced,and cardiac muscle fibers and intercalated disc ultrastructure were both damaged in Cd group; however,those in Cd+ As Ⅳ were improved when compared with Cd group.Conclusion:Cd can damage cardiomyocyte intercalated disc ultrastructure,and reduce expression of Cx43; astragaloside Ⅳ retains the expression level of Cx43 and maintains morphologic integrity of cardiomyocyte intercalated disc.关键词
黄芪甲苷/镉/心肌细胞/连接蛋白43/超微结构Key words
astragaloside Ⅳ/ cadmium/ cardiomyocyte/ connexin 43/ ultrastructure引用本文复制引用
姚志勇,廖晓岗,宁巍,王红,范京川,李兆飞..急性染镉对大鼠心肌细胞闰盘超微结构和连接蛋白43表达的影响及黄芪甲苷的拮抗作用[J].解剖学杂志,2013,36(4):732-735,767,5.
