解剖学杂志2013，Vol.36Issue(4)：775-778,863,5.DOI:10.3969/j.issn.1001-1633.2013.04.018
初级纤毛在白藜芦醇诱导大鼠骨髓基质细胞分化为神经元样细胞中的作用
Effect of primary cilia on differentiation of rat bone marrow stromal cells into neuron-like cells induced by resveratrol in vitro
摘要
Abstract
Objective:To study the effect of primary cilia on differentiation of rat bone marrow stromal cells (MSCs) into neuron-like cells induced by resveratrol in vitro.Methods:Rat MSCs from the whole bone marrow were cultured,purified by adherent method in vitro and induced to differentiate into neuron-like cells with serum-free DMEM/F12 containing 15μmol/L resveratrol.Cell morphology was observed under an invert microscope.The protein expressions of neuron specific enolase (NSE) and microtubule associated protein-2 (MAP-2) were detected by western blotting.The proliferation of MSCs was detected by immunofluorescence.Scanning electron microscopy and immunofluorescence was used to detect primary cilia.Results:After induced by resveratrol,60％ of the cells took on neuron-like morphology with the cell body shrinkage,threedimensional sense enhancement.Western blotting confirmed that almost all MSCs and the control group cells were poorly positive for NSE and were positive for expressions of NSE and MAP-2 in the resveratrol-induced group.With the prolongation of induction,the expressions of NSE and MAP-2 increased gradually.After starvation for 24h,90％ MSCs entered in the resting state of growth.Scanning electron microscopy and immunofluorescence showed that MSCs in the resting state of growth possessed primary cilia,and resveratrol induced MSCs to differentiate into neuron-like cells at the moment.However,the control group was no markedly changed.Conclusion:Resveratrol can induce MSCs to differentiate into neuron-like cells.Moreover,primary cilia may play an important role for neuronal differentiation of MSCs.关键词
白藜芦醇/初级纤毛/骨髓基质细胞/神经元样细胞/细胞分化Key words
resveratrol/ primary cilia/ bone marrow stromal cells/ neuron-like cells/ cell differentiation引用本文复制引用
黄家贵,沈长波,刘舒,徐兰,杨琴..初级纤毛在白藜芦醇诱导大鼠骨髓基质细胞分化为神经元样细胞中的作用[J].解剖学杂志,2013,36(4):775-778,863,5.基金项目
国家自然科学基金面上项目(81071119) （81071119）
重庆市卫生局科研基金(2009-2-359) （2009-2-359）
