解剖学杂志2013，Vol.36Issue(4)：794-799,6.DOI:10.3969/j.issn.1001-1633.2013.04.023
表皮生长因子受体沉默联合尼美舒利对胶质瘤细胞U251作用及机制
Effect and mechanism of silencing of epidermal growth factor receptor combined with nimesulide on glioma cell line U251
摘要
Abstract
Objective:To investigate the proliferation-inhibiting and induce the apoptosis by combination with nimesulide (NIM) and silencing epidermal growth factor (EGF) receptor on glioma cells U251.Methods:Human U251 glioma cells were incubated by combination with NIM(0,25,50,100,200,400 μmol/L) and inhibiting RNA targeted EGF receptor (siRNAEGFR) for 12,24,48 and 72 h.The vascular endothelial growth factor (VEGF) level in the culture supernatant was quantitated by ELISA assay,and the growth inhibition rate of U251 was investigated by MTT assay.The cell cycle and apoptosis were tested by flow cytometry; The cell proliferation,apoptosis and angiopoiesis related proteins were tested by Western blotting.Results:The combination of NIM and siRNA-EGFR inhibited the growth of U251 cells more effectively in a concentration-time dependent way than did siRNA-EGFR or COX-2 inhibitor.The combination with siRNA-EGFR and NIM showed a dose-dependent increase in G2/M phase arrest and apoptosis,and up-regulated the expression of Bax and downregulated the expression of bcl-2,VEGF,ANG-2.Conclusion:Cell growth inhibition rates were significantly increased by the combination of the NIM and siRNA-EGFR played additive action,mechanism of which is involved in the up-regulation of the expression of Bax,and down-regulation of the expression of bcl-2,VEGF and ANG-2.关键词
尼美舒利/胶质瘤/U251细胞/细胞凋亡/表皮生长因子受体沉默Key words
nimesulide/ glioma/ U251 cells/ cell apoptosis/ silence to epidermal growth factor receptor引用本文复制引用
戴国栋,游兴松,袁玉林..表皮生长因子受体沉默联合尼美舒利对胶质瘤细胞U251作用及机制[J].解剖学杂志,2013,36(4):794-799,6.基金项目
湖北省自然科学基金(301131485) （301131485）
