实用临床医药杂志2013，Vol.17Issue(16)：15-17,38,4.DOI:10.7619/jcmp.201316006
青海地区结核分枝杆菌检测与KatG、rpoB及gyrA的突变特征分析
Detection of mycobacterium tuberculosis in Qinghai area and analysis of gene mutation characteristics of KatG, rpoB and gyrA
摘要
Abstract
Objective To explore the drug resistance of mycobacterium tuberclosis (MTB)in Qinghai area to RFP,fluoroquinolones and INH and their relationship with the corresponding rpoB,gyrA,katG gene mutations.Methods PCR-DNA method was used to detect gene sequences of gyrA,katG and rpoB isolated by mycobacterium tuberculosis.Results After testing,katG gene mutation rate found in the resistant INH strain was70.9 ％.rpoB gene mutation rate in the resistant RFP strain was 83.33 ％.gyrA genemutation rate in fluoroquinolone resistant strains was 77.27 ％.KatG mutation was the most common form of Ser315Thr (86.67％).The most common mutant forms of rpoB were amino acids 526 and 531,and the total mutation rate was 72.73％.Fluoroquinolones mutation occurred mainly in region decided by gyrA gene (QRDR) mutations,and the main point was in 94 (65 ％).Conclusion Gene mutations of KatG,gyrA and rpoB are major molecular mechanism for drug resistance of mycobacterium tuberclosis (MTB) in Qinghai area to RFP,fluoroquinolones and INH.The PCR-DNA sequencing method is easy to apply and it can be used as assistant drug resistance in rapid detection of mycobacterium tuberculosis INH,fluoroquinolones and RFP in clinical practice.关键词
耐药性/基因突变/结核分枝杆菌Key words
drug resistance/ gene mutation/ mycobacterium tuberculosis分类
医药卫生引用本文复制引用
王芝,马如存,卓玛,常凤霞,郝娟,王玉清..青海地区结核分枝杆菌检测与KatG、rpoB及gyrA的突变特征分析[J].实用临床医药杂志,2013,17(16):15-17,38,4.基金项目
青海省科技厅资料项目(2011-YJ1438) （2011-YJ1438）
