生物灾害科学Issue(2):165-169,5.DOI:10.3969/j.issn.2095-3704.2012.02.009
柑橘黄龙病PCR检测技术研究
Study on PCR Detection of Citrus Huanglongbing Bacterium
摘要
Abstract
Specific primer pairs fA2/rJ5 based on the β-operon of Candidatus Liberobacter asiaticum was used for PCR detection of citrus Huanglongbing bacterium, The result was depended on whether the target DNA fragment was existed. This PCR protocol is efficient, convenient and sensitive for early detection of citrus Huanglongbing bacterium, which was very important for the control of the spread of the disease.关键词
柑橘黄龙病/β-operon/PCRKey words
Citrus Huanglongbing/β-operon/polymerase chain reaction分类
农业科技引用本文复制引用
张伟..柑橘黄龙病PCR检测技术研究[J].生物灾害科学,2012,(2):165-169,5.基金项目
河南省科技厅基础前沿项目(092300410244) (092300410244)