姜黄素对口腔鳞癌细胞中细胞骨架及Rho/ROCK信号通路的影响OACSTPCD
Effects of Curcumin on the Cytoskeleton of OSCC Cells by Rho/ROCK Pathway
目的:探讨姜黄素对口腔鳞癌细胞系HN13细胞骨架的影响及其相关的分子机制.方法:用考马斯亮蓝染色分析法检测不同浓度姜黄素(0、10、20、40lμmol/L)作用24h的细胞,观察姜黄素对鳞癌细胞骨架的影响.用免疫组织化学法半定量检测不同浓度姜黄素(0、10、20、40 μmol/L)作用24h后细胞内F-actin含量的变化.用Western印迹法检测不同浓度姜黄素(0、10、20、40 μmol/L)作用24 h后ROCK2及p-ROCK2蛋…查看全部>>
Objective:The present article aimed to study whether the effects of curcumin are related to cytoskeleton variation in OSCC cell line HN13 and its mechanism.Methods:HN13 cells were treated and exposed with different concentrations of curcumin (0,10,20,40 μmol/L).After 24 h,Coomassie blue staining was used to measure the cystoskeleton o f HN13 cells.Immunohistochemistry was used to observe the expression of F-actin.The expression of ROCK2 and phosphated ROCK2 …查看全部>>
范德生;甄蕾;郭姜莉
上海中医药大学附属曙光医院病理科,上海200021上海市口腔病防治院口腔内科,上海200001上海市口腔病防治院口腔内科,上海200001
医药卫生
Rho/ROCK信号通路姜黄素鳞癌细胞骨架
Rho/ROCK2 pathway curcumin oral squamous cell carcinoma cystoskeleton
《口腔颌面外科杂志》 2013 (3)
182-185,4
上海市青年医师培养资助计划(人才-2012-04-03)
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