林产化学与工业2013，Vol.33Issue(4)：15-21,7.DOI:10.3969/j.issn.0253-2417.2013.04.003
马尾松针叶聚戊烯醇体外和体内抗氧化活性研究
Antioxidant Activity of Polyprenols from Pine Needles of Pinus massoniana L.in vitro and in vivo
摘要
Abstract
The present study was undertaken to evaluate the antioxidant activity of polyprenols from pine needles of Pinus massoniana L.in vitro and in vivo.In vitro antioxidant activities of polyprenols were evaluated by determining their ability to scavenge free radicals and inhibit lipid peroxidation and mitochondrial swelling.In vivo antioxidant activities were determined by their effect on antioxidant enzymatic activities in serum,heart,and liver.The results showed that polyprenols exhibited concentration-dependent inhibition of lipid peroxidation and mitechondrial swelling at the 12.5-100 mg/L.It displayed high scavenging capacity towards the hydroxyl,superoxide and DPPH radicals in a dose-dependent manner and the median inhibitory concentration (IC5o) were 34.87,27.13 and 58.23 mg/L,respectively.Moreover,polyprenols could significantly increase the activities of SOD and GSH-Px while decrease the formation of MDA in serum,liver and heart (p ＜ 0.05).Polyprenols from P.massoniana L.have excellent antioxidant activities and might be a valuable antioxidant of natural source applicable in the medicine industry.关键词
松针/聚戊烯醇/抗氧化/脂质过氧化/清除自由基Key words
pine needles/ polyprenols / antioxidant/ lipid peroxides / radical-scavenging activity分类
化学化工引用本文复制引用
郑光耀,何玲,高丽萍,连燕霞,谢衡,薄采颖,李若达..马尾松针叶聚戊烯醇体外和体内抗氧化活性研究[J].林产化学与工业,2013,33(4):15-21,7.基金项目
科技部农业科技成果转化资金项目(2008GB24320412) （2008GB24320412）
广东省重大科技专项(2012A080201005) （2012A080201005）
"十二五"国家重大新药创制专项(2012ZX09504001-001) （2012ZX09504001-001）
