临床口腔医学杂志2013，Vol.29Issue(8)：460-463,4.DOI:10.3969/j.issn.1003-1634.2013.08.005
束缚应激对Pg-LPS诱导的大鼠腹腔巨噬细胞释放细胞因子的影响
Effects of restain stress and Pg-LPS on the change of cytokines secretion from peritoneal macrophages in rats
李榕卿 1林实 2李艳芬 2赵欣2
作者信息
- 1. 南京军区福州总院四七六临床部口腔科 福建福州 350007
- 2. 福建医科大学附属口腔医院 福建福州 350002
- 折叠
摘要
Abstract
Objective:To explore the effects of restrain Pg-LPS and stress on the change of cytokines secretion from peritoneal macrophages in rats.Method:Rats (SD,male) were tested by using restrain stress.Rats were divided into 3 experimental groups randomly (no stress,one day stress,12 days stress).After stress conditions,the rats were sacrificed,and their peritoneal macrophages were isolated,cultured and purify.All the cells were then cultured with RPMI-1640+1 μg/mL Pg-LPS.After 24 hours,cell culture supernatants were collected and assayed for IL-Iβ、IL-2、IL-6、IL-10.Result:The number of cells in the peritoneal cavity of 12 days stressed rats was significantly reduced in comparison to those from non-stressed animals (P ＜0.05).The level of cytokines was no significant difference between the one day stress and no stress group (P ＞0.05).The level of IL-1β、IL-6 is suggested significant difference between the 12 days stress and no stress group (P ＜0.05).The level of IL-2 is suggested significant difference between the 12 days stress and 1 day stress group (P ＜0.05).The level of IL-10 is suggested no significant difference within groups (P ＞0.05).Conclusion:The results suggest chronic restrain stress can reduce the number of macrophages in the peritoneal cavity in rats.Chronic restrain stress modulates cytokine secretion from Pg-LPS stimulated macrophages.关键词
Pg-LPS/应激/巨噬细胞/细胞因子Key words
Pg-LPS/ stress/ macrophage/ cytokines分类
生物科学引用本文复制引用
李榕卿,林实,李艳芬,赵欣..束缚应激对Pg-LPS诱导的大鼠腹腔巨噬细胞释放细胞因子的影响[J].临床口腔医学杂志,2013,29(8):460-463,4.
