临床神经病学杂志2013，Vol.26Issue(4)：278-281,4.
美金刚联合丰富环境对快速衰老小鼠海马脑源性神经营养因子、酪氨酸蛋白激酶受体B表达及学习记忆能力的影响
Effects of Memantine combined with enriched environment on the expression of brain derived neurophic factor and tyrosine kinase receptor B in hippocampus and the learning and memory ability of senescence accelerated mouse prone 8
摘要
Abstract
Objective To explore the effects of Memantine combined with enriched environment (EE) on the expression of brain derived neurophic factor (BDNF) and tyrosine kinase receptor B (TrkB) in hippocampus and the learning,memory ability of senescence accelerated mouse prone 8 (SAMP8).Methods Twenty-four male SAMP8 mouse were randomly divided into control group,EE group,Memantine group and Mematine combined with EE group (ME group),6 mouse in each group.After 8-week-treatment,Morris water maze test was performed to evaluate improvement of spatial learning,memory ability.The expression of BDNF and TrkB was detected by Western blot and Real time PCR.Results Compared with SE group,the overall mean escape latency of EE group and Memantine group was significantly shorten since the third day,and which of EM group was shorten since the second day (P ＜0.05-0.01) ; and the mean escape latency of EM group was shorter than EE and Memantine groups in the fourth day (all P ＜ 0.05).Compared with control group,the frequencies of crossing platform in the other 3 groups were significantly increased (P ＜ 0.05-0.01) ; and the frequencies of crossing platform in the ME group was more than EE and Memantine groups (all P ＜ 0.01).Compared with control group,the levels of BDNF mRNA,protein and TrkB protein in the other 3 groups were significantly increased (P ＜ 0.05-0.01) ; and which in ME group were significantly higher than the EE and Memantine groups (P ＜ 0.05-0.01).However,the difference of levels of TrkB mRNA between each group was no statistical significance.Conclusions The Memantine and EE can improve the learning,memory ability of SAMP8 mouse,and increase the expression of the BDNF and TrkB in hippocampus.The effects can be more significant when Memantine combined with EE.关键词
快速衰老小鼠/美金刚/丰富环境/脑源性神经营养因子/酪氨酸蛋白激酶受体BKey words
senescence accelerated mouse prone 8/ Memantine/ enriched environment/ brain derived neurophic factor/ tyrosine kinase receptor B分类
医药卫生引用本文复制引用
杜明洋,管青山,王小姗..美金刚联合丰富环境对快速衰老小鼠海马脑源性神经营养因子、酪氨酸蛋白激酶受体B表达及学习记忆能力的影响[J].临床神经病学杂志,2013,26(4):278-281,4.基金项目
江苏省自然科学基金(BK2008081) （BK2008081）
南京医科大学科技发展基金(2010NJMUZ44) （2010NJMUZ44）
